Summary
Type: SPE
Application #: 305400
Catalog#: 730130 - SPE Cartridges, Chromabond, C18, 6ml, PP, 2000mg 30/pk
Detection:
Conditions: Column conditioning: 6 mL methanol, then 6 mL water
(Do not let run the column dry.)
Sample aspiration: The pretreated sample is loaded onto the column
Washing: 6 mL 5% methanol
Elution 1: 18 mL 30 % methanol
Elution 2: 12 mL 50 % methanol
Elution 3: 6 mL methanol
Evaporation: to dryness of the seperately collected fractions
Authors: Fan, P. et al.
Source: J. Sep. Sci. 2009, 32, 2979–2984